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dedicated imagej plugin  (Nikon)


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    Structured Review

    Nikon dedicated imagej plugin
    Dedicated Imagej Plugin, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1626 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imagej+plugin/ECLIPSE+Ts2R/pm41780697-118-15-8
    Average 99 stars, based on 1626 article reviews
    dedicated imagej plugin - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    other:

    Article Title: A disease-associated PPP2R3C-MAP3K1 phospho-regulatory module controls centrosome function.
    Article Snippet: RPE1-hTERT (RPE1), SKNBE2, and HEK293T cells were obtained from ATCC.

    Article Title: The nanoscale molecular morphology of docked exocytic dense-core vesicles in neuroendocrine cells
    Article Snippet: Localization data were processed using both the ThunderStorm ImageJ plugin and Nikon Elements 4.1.

    Light Microscopy:

    Article Title: Refined model of common peroneal nerve injury and regeneration: A neurophysiological, morphometric, and functional analysis in the adult rat.
    Article Snippet: Muscles were formalin fixed for 24 h, paraffin embedded, cut to 8 μm (Leica Ultracut RM 22359) and NADH stained for 15 min at 37 ◦C (Tris buffer, NBT, NADH). .. Muscle fiber area was recorded using light microscopy (Nikon Eclipse Ts2R) and measurement via a dedicated ImageJ plugin (Image J plugin MyoSAT Version 3.5, Cheetham Lab, Department of Biomedical Science, Ohio University, Athens, Ohio, USA). .. For statistical analysis, all data were collected in Microsoft® Excel® (Microsoft 365 MSO, Version 2505), analyzed and visualized with GraphPad Prism (Version 10.5.0 774).

    Software:

    Article Title: Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
    Article Snippet: .. Accordingly, we have switched from the ImageJ plugin FairSIM to the Nikon Elements 12 software, which offers greater control over reconstruction. ..

    Article Title: The actin networks of chytrid fungi reveal evolutionary loss of cytoskeletal complexity in the fungal kingdom.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Penicillin/streptomycin Thermo-Fisher Cat#P4333-20ML Concanavalin A Sigma Cat#C2010 LatrunculinB Millipore Cat#4280201MG CK666 Calbiochem/Sigma Cat#182515 CK689 Calbiochem/Sigma Cat#182517 SMIFH2 Tocris Bioscience Cat#440110 DAPI Life Technologies Cat#D1306 Alexa Fluor488 Phalloidin Life Technologies Cat#A12379 Polyethyleneimine Sigma Cat#P3143 Chitinase Sigma Cat#C6137-5UN Experimental models: organisms/strains Batrachochytrium dendrobatidis strain JEL423 Joyce Longcore JEL423 Batrachochytrium salamandrivorans isolate AMFP1 Frank Pasmans AMFP1 Spizellomyces punctatus Koch type isolate NG-3 ATCC 48900 Rhizoclosmatium globosum strain JEL800 Tim James (https://czeum.herb.lsa.umich.edu/) JEL800 Software and algorithms Basic Local Alignment Search Tool (BLAST) 67 https://blast.ncbi.nlm.nih.gov/Blast.cgi Position Specific Iteration-BLAST (PSI-BLAST) 68 https://blast.ncbi.nlm.nih.gov/Blast.cgi UniprotKB https://www.uniprot.org/ N/A HMMER suite v3.2.1 http://Hmmer.org http://Hmmer.org Pfam database v32 69 http://pfam.xfam.org European Bioinformatics Institute (EMBL-EBI) https://www.ebi.ac.uk/ https://www.ebi.ac.uk/ Coiled-Coil predictor 70 N/A Phobius program 71 N/A CD-hit program 72,73 N/A IQTree webserver 74 http://iqtree.cibiv.univie.ac.at/ iTOL v5.5.1 75 https://itol.embl.de/ FIJI 76 https://imagej.net/Fiji/Downloads CellCounter FIJI plugin Kurt De Vos https://imagej.nih.gov/ij/plugins/ cell-counter.html Autoquant X3 vX3.1.3 Media Cybernetics https://www.mediacy.com/79- products/autoquant-x3 NIS Elements w/ Advanced Research Package Nikon https://www.microscope.healthcare. nikon.com/products/software/niselements/nis-elements-advancedresearch Other Batrchochytirum dendrobatidis JAM81 reference assembly v1.0 US DOE Joint Genome Institute NCBI: GCA_000203795.1 Batrachochytirum dendrobatidis JEL423 assembly Broad Institute NCBI: GCA_000149865.1 Batrachochytrium salamandrivorans representative assembly (assembly Batr_sala_BS_V1) Broad Institute NCBI: GCA_002006685.1 Spizellomyces punctatus DAOM BR117 representative assembly (S_punctatus_V1) Broad Institute NCBI: GCA_000182565.2 (Continued on next page) e1 Current Biology 31, 1192–1205.e1–e6, March 22, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rhizoclosmatium globosum JEL800 representative assembly (Rhihy1) DOE Joint Genome Institute NCBI: GCA_002104985.1 Allomyces macrogynus ATCC 38327 representative assembly (A_macrogynus_V3) Broad Institute NCBI: GCA_000151295.1 Saccharomyces cerevisiae S288C NCBI RefSeq build R64 assembly Saccharomyces Genome Database NCBI: GCF_000146045.2 Schizosaccharomyces pombe strain 972 NCBI RefSeq assembly S. pombe European Sequencing Consortium (EUPOM) NCBI: GCF_000002945.2 Schizosaccharomyces japonicus strain yFS275 NCBI RefSeq assembly Broad Institute NCBI: GCF_000149845.2 Candida albicans strain SC5314 NCBI RefSeq assembly (assembly ASM18296v3) Stanford University NCBI: GCA_000182965.3 Aspergillus nidulans strain FGSC A4 NCBI RefSeq assembly (assembly ASM14920v2) Broad Institute NCBI: GCA_000149205.2 Magnaporthe oryzae strain 70-15 NCBI RefSeq assembly (Pyricularia oryzae 70-15 assembly MG8)) International Rice Blast Genome Consortium NCBI: GCA_000002495.2 Neurospora crassa strain OR74A NCBI RefSeq assembly (assembly NC12) Broad Institute NCBI: GCA_000182925.2 Ustilago maydis strain 512 NCBI RefSeq assembly (assembly Umaydis521_2.0) Broad Institute NCBI: GCA_000328475.2 Dictyostelium discoideum AX4 NCBI Reference assembly (assembly dicty2.7) The Dictyostelium discoideum Sequencing Consortium NCBI: GCA_000004695.1 Human reference genome on NCBI (assembly GRCh38.p13) Genome Reference Consortium NCBI: GCA_000001405.28 Mus musculus reference genome on NCBI (assembly GRCm39) Genome Reference Consortium NCBI: GCF_000001635.27 Arabidopsis thaliana reference genome on NCBI (assembly TAIR10.1) The Arabidopsis Information Resource NCBI: GCA_000001735.2 Oryctolagus cuniculus reference genome on NCBI (assembly OryCun2.0) The Genome Sequencing Platform, The Assembly Computation and Development Core Team NCBI: GCA_000003625.1 Giardia intestinalis representative genome on NCBI (assembly UU_WB_2.1) Marine Biological Laboratory NCBI: GCA_000002435.2 Chlamydomonas reinhardtii representative genome on NCBI (assembly v3.0) DOE Joint Genome Institute NCBI: GCA_000002595.2 Neocallimastix californiae G1 genome 53 https://mycocosm.jgi.doe.gov/Neosp1/ Neosp1.home.html Orpinomyces sp. strain C1A v1.0 genome 53 https://mycocosm.jgi.doe.gov/Orpsp1_ 1/Orpsp1_1.home.html Chytriomyces hyalinus JEL632 v1.0 genome 54 https://mycocosm.jgi.doe.gov/Chyhya1/ Chyhya1.home.html ll OPEN ACCESSArticle

    Control:

    Article Title: Turn-key mapping of cell receptor force orientation and magnitude using a commercial structured illumination microscope
    Article Snippet: .. Accordingly, we have switched from the ImageJ plugin FairSIM to the Nikon Elements 12 software, which offers greater control over reconstruction. ..

    Recombinant:

    Article Title: The actin networks of chytrid fungi reveal evolutionary loss of cytoskeletal complexity in the fungal kingdom.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins Penicillin/streptomycin Thermo-Fisher Cat#P4333-20ML Concanavalin A Sigma Cat#C2010 LatrunculinB Millipore Cat#4280201MG CK666 Calbiochem/Sigma Cat#182515 CK689 Calbiochem/Sigma Cat#182517 SMIFH2 Tocris Bioscience Cat#440110 DAPI Life Technologies Cat#D1306 Alexa Fluor488 Phalloidin Life Technologies Cat#A12379 Polyethyleneimine Sigma Cat#P3143 Chitinase Sigma Cat#C6137-5UN Experimental models: organisms/strains Batrachochytrium dendrobatidis strain JEL423 Joyce Longcore JEL423 Batrachochytrium salamandrivorans isolate AMFP1 Frank Pasmans AMFP1 Spizellomyces punctatus Koch type isolate NG-3 ATCC 48900 Rhizoclosmatium globosum strain JEL800 Tim James (https://czeum.herb.lsa.umich.edu/) JEL800 Software and algorithms Basic Local Alignment Search Tool (BLAST) 67 https://blast.ncbi.nlm.nih.gov/Blast.cgi Position Specific Iteration-BLAST (PSI-BLAST) 68 https://blast.ncbi.nlm.nih.gov/Blast.cgi UniprotKB https://www.uniprot.org/ N/A HMMER suite v3.2.1 http://Hmmer.org http://Hmmer.org Pfam database v32 69 http://pfam.xfam.org European Bioinformatics Institute (EMBL-EBI) https://www.ebi.ac.uk/ https://www.ebi.ac.uk/ Coiled-Coil predictor 70 N/A Phobius program 71 N/A CD-hit program 72,73 N/A IQTree webserver 74 http://iqtree.cibiv.univie.ac.at/ iTOL v5.5.1 75 https://itol.embl.de/ FIJI 76 https://imagej.net/Fiji/Downloads CellCounter FIJI plugin Kurt De Vos https://imagej.nih.gov/ij/plugins/ cell-counter.html Autoquant X3 vX3.1.3 Media Cybernetics https://www.mediacy.com/79- products/autoquant-x3 NIS Elements w/ Advanced Research Package Nikon https://www.microscope.healthcare. nikon.com/products/software/niselements/nis-elements-advancedresearch Other Batrchochytirum dendrobatidis JAM81 reference assembly v1.0 US DOE Joint Genome Institute NCBI: GCA_000203795.1 Batrachochytirum dendrobatidis JEL423 assembly Broad Institute NCBI: GCA_000149865.1 Batrachochytrium salamandrivorans representative assembly (assembly Batr_sala_BS_V1) Broad Institute NCBI: GCA_002006685.1 Spizellomyces punctatus DAOM BR117 representative assembly (S_punctatus_V1) Broad Institute NCBI: GCA_000182565.2 (Continued on next page) e1 Current Biology 31, 1192–1205.e1–e6, March 22, 2021 .. REAGENT or RESOURCE SOURCE IDENTIFIER Rhizoclosmatium globosum JEL800 representative assembly (Rhihy1) DOE Joint Genome Institute NCBI: GCA_002104985.1 Allomyces macrogynus ATCC 38327 representative assembly (A_macrogynus_V3) Broad Institute NCBI: GCA_000151295.1 Saccharomyces cerevisiae S288C NCBI RefSeq build R64 assembly Saccharomyces Genome Database NCBI: GCF_000146045.2 Schizosaccharomyces pombe strain 972 NCBI RefSeq assembly S. pombe European Sequencing Consortium (EUPOM) NCBI: GCF_000002945.2 Schizosaccharomyces japonicus strain yFS275 NCBI RefSeq assembly Broad Institute NCBI: GCF_000149845.2 Candida albicans strain SC5314 NCBI RefSeq assembly (assembly ASM18296v3) Stanford University NCBI: GCA_000182965.3 Aspergillus nidulans strain FGSC A4 NCBI RefSeq assembly (assembly ASM14920v2) Broad Institute NCBI: GCA_000149205.2 Magnaporthe oryzae strain 70-15 NCBI RefSeq assembly (Pyricularia oryzae 70-15 assembly MG8)) International Rice Blast Genome Consortium NCBI: GCA_000002495.2 Neurospora crassa strain OR74A NCBI RefSeq assembly (assembly NC12) Broad Institute NCBI: GCA_000182925.2 Ustilago maydis strain 512 NCBI RefSeq assembly (assembly Umaydis521_2.0) Broad Institute NCBI: GCA_000328475.2 Dictyostelium discoideum AX4 NCBI Reference assembly (assembly dicty2.7) The Dictyostelium discoideum Sequencing Consortium NCBI: GCA_000004695.1 Human reference genome on NCBI (assembly GRCh38.p13) Genome Reference Consortium NCBI: GCA_000001405.28 Mus musculus reference genome on NCBI (assembly GRCm39) Genome Reference Consortium NCBI: GCF_000001635.27 Arabidopsis thaliana reference genome on NCBI (assembly TAIR10.1) The Arabidopsis Information Resource NCBI: GCA_000001735.2 Oryctolagus cuniculus reference genome on NCBI (assembly OryCun2.0) The Genome Sequencing Platform, The Assembly Computation and Development Core Team NCBI: GCA_000003625.1 Giardia intestinalis representative genome on NCBI (assembly UU_WB_2.1) Marine Biological Laboratory NCBI: GCA_000002435.2 Chlamydomonas reinhardtii representative genome on NCBI (assembly v3.0) DOE Joint Genome Institute NCBI: GCA_000002595.2 Neocallimastix californiae G1 genome 53 https://mycocosm.jgi.doe.gov/Neosp1/ Neosp1.home.html Orpinomyces sp. strain C1A v1.0 genome 53 https://mycocosm.jgi.doe.gov/Orpsp1_ 1/Orpsp1_1.home.html Chytriomyces hyalinus JEL632 v1.0 genome 54 https://mycocosm.jgi.doe.gov/Chyhya1/ Chyhya1.home.html ll OPEN ACCESSArticle

    Generated:

    Article Title: The GalNAc-T Activation (GALA) Pathway: Drivers and markers.
    Article Snippet: .. To analyse images provided by Tabak’s group, an ImageJ plugin (Nikon ND2 Reader) was used to export z-stacks of each channel in . avi format and the maximum projection was generated from each z-stack. ..

    Article Title: The GalNAc-T Activation (GALA) Pathway: Drivers and markers
    Article Snippet: .. To analyse images provided by Tabak’s group, an ImageJ plugin (Nikon ND2 Reader) was used to export z-stacks of each channel in .avi format and the maximum projection was generated from each z-stack. ..



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    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the <t>ImageJ</t> <t>plugin</t> Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the <t>LPX</t> ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.
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    Image Search Results


    Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Journal: eBioMedicine

    Article Title: SLK is mutated in individuals with a neurodevelopmental disorder

    doi: 10.1016/j.ebiom.2025.105725

    Figure Lengend Snippet: Quantification of wound assay, Golgi appearance and microtubule distribution in patient-derived fibroblasts compared to control . (a) Representative images of sub-confluent fibroblast cells derived from either the index individual in Family 1 (IV:1) or control cell lines at 0 h of wound-induced cell migration assay. Scale bar: 1000 μm. (b) Wound closure was followed serially for 29 h during which a significant difference in cell migration was observed between the two cell lines. Scale bar: 1000 μm. (c) Percent wound closure was evaluated up to 29 h. (d) The remaining area of the wound was measured in μm 2 . The percent wound closure and area of the wound were measured using the ImageJ plugin Wound healing size tool image tool analysis ( https://github.com/AlejandraArnedo/Wound-healing-size-tool/wiki ). Data from 50 cells were collected and analysed. (e) Control cells showing perinuclear compact and polarized Golgi apparatus that are directed toward the migrating wound edge. (f) IV:1-derived fibroblast cells depicting a dispersed and non-polarized (oriented) Golgi apparatus towards the migrating/wound edge. (g) A representative image of normally polarized and parallel orientated microtubules in control cells. (h) A representative image of individual IV:1-derived fibroblast cells showing an un-polarized chaotic microtubule distribution; microtubules labelled with α-tubulin (green) and the centrosome is labelled with Pericentrin (orange) antibodies. (i) The total area of Golgi from 100 cells was measured using the ImageJ software. Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells. Data from 50 cells were collected and analysed and p-value was calculated based on Welch's t-test for (c, d, i, j and k). The red squares represent datapoints collected from control sample, the green circles represent datapoints collected from the patient sample (IV:1). The horizontal line represents the mean.

    Article Snippet: Error bars represent stdev. (j) The directional orientation of Golgi from 100 cells was assessed and presented as percent mean polarization with error bars representing stdev of 3 replicates. (k) The direction of orientation (paralellness) of microtubules was measured using the LPX ImageJ plugin ( https://lpixel.net/services/research/lpixel-imagej-plugins/ ) ; and data presented as mean and stdev of images of 100 cells.

    Techniques: Derivative Assay, Control, Cell Migration Assay, Migration, Software